Effect of calcination treatment on the in vitro bioactivity of ceramic powders
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https://doi.org/10.69717/jaest.v6.i3.168Keywords:
Calcium phosphate, Calcination, Bioactivity, Physiological solution, Bioceramic powder, Tissue engineering, Calcium phosphate, Calcination, Bioactivity, Physiological solution, Bioceramic powder, Tissue engineeringAbstract
The aim of this study was to evaluate the effect of varying calcination temperature and duration (500°C -1h, 600°C-1h, 600°C-6h, and 900°C-3h) on the bioactivity property of crystalline hydroxyapatite pellets immersed in a physiological solution (simulated body fluid) for 7 days. The microstructural properties and composition of the powders−such as the degree of crystallinity, crystallite size, additional thermal phases of -tricalcium phosphate (β-TCP) and CaO, and functional groups (hydroxyl and carbonate)−vary depending on the calcination parameter and immersion process. X-ray diffraction (XRD) and infrared spectroscopy (IR) analyses showed significant microstructural changes for the HAP calcined at 900°C for 3 hours. Moreover, atomic absorption spectroscopy (AAS) analysis and pH measurements indicated variations in calcium ion concentration (42.2 ± 0.70 mg/L after 3 days, and 34.2 ± 0.48 mg/L after 7 days of immersion time) and pH of the medium-which decreased from 7.440 ± 0.02 to 7.305 ± 0.03-for the HAP pellet calcined at 900°C. These results were confirmed by X-ray fluorescence (XRF) and electron microscopy, which indicated an increase in the Ca content from 55.06 ± 0.156% to 55.85±0.148%, and in the P content from 24.64±0.155% to 27.59±0.158%, as well as the deposition of dispersed particles and large agglomerates, suggesting the formation of a non-uniform layer of calcium phosphate (CaP) on the surface of the powder pellet calcined at 900°C.
Highlights
- Variations in the calcination parameters of hydroxyapatite affect its biological response.
- CaP deposit formation after 7 days of immersion on the surface of HAp calcined at 900°C.
- The thermal decomposition of HAp can promote the formation of CaP deposits.
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References
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